New York Hospital / Cornell University Medical Center Laboratory of Urological Oncology

Adapted from Monoclonal Antibodies by Karol Sikora and Howard M. Smedley

Scanning technique

If an antibody can be prepared which is capable of recognizing tumor cells, but has little or no cross-reaction to any normal human tissue, it may be suitable for scanning. In principle a radioactive isotope may be attached to a monoclonal antibody is such a fashion that the immunoreactivity of the antibody, as defined by in vitro biding assays, is unaltered. If such a labelled antibody is then injected into the patient, after a suitable time the antibody will bind to tumor associated antigens in the patient. The localization of this antibody-antigen complex can be detected by an external radiation camera and an image produced of the patient's body outline with the position of the labelled antibody-antigen complexes. It is correct to think of such scans as antigen distribution images where the molecule being imaged is in fact the tumor-associated antigen.


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