Fig. 3 IEM of The Internalized mAb J591

Figure 3. Immunoelectron microscopy of internalized mAb J591 in LNCaP cells. Cells were incubated with J591 at 370C for 10 min (A,B, and C) or 2 hrs (D) and processed for immunogold labeling as described in Materials and Methods. Note the accumulation of gold particles in clathrin coated vesicles (A and B) and in vesicles proximal to the plasma membrane (C). At 2 hrs note the accumulation of gold particles in a juxtanuclear region (arrowheads). N=Nucleus. Bars correspond to 34nm (A), 65nm (B and C) and 85nm (D), respectively.